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Image Search Results
Journal: The EMBO Journal
Article Title: Compensatory endocytosis in bladder umbrella cells occurs through an integrin-regulated and RhoA- and dynamin-dependent pathway
doi: 10.1038/emboj.2010.91
Figure Lengend Snippet: Dependence of CE on dynamin. (A) Localization of dynamin-2 in uroepithelial tissue. The apical surface of an umbrella cell is indicated by arrows. (B) Effect of dynasore on CT during filling and voiding. Voiding was initiated at the arrow. The boxed region in the left panel is expanded in the right-hand panel. Control data are reproduced from Figure 1A. Values are mean±s.e.m. (n⩾5), and those values significantly different than control samples (P<0.5) are indicated with an asterisk. (C) Uptake of FITC-WGA and FITC-dextran in tissue treated with dynasore (250 μM). (D) Quantitation of FITC-WGA and FITC-dextran uptake in tissue treated with MβCD (10 mM), cyto-D (25 μg/ml), C3 toxin (1 μg/ml), dynasore (250 μM), or Y-27632 (25 μM). Values are mean±s.e.m. (n⩾10), and those values significantly different than control samples (P<0.05) are indicated with an asterisk. (E, F) Rat bladders were transduced in situ with adenovirus expressing GFP alone or GFP-labelled DN-dynaminK44A (DN-dynamin). The bladder was filled with Alexa647-WGA, stimulated to undergo voiding, excised, and then fixed and processed for immunofluorescence. (E) A transduced cell expressing DN-dynaminK44A (K44A) is indicated with an asterisk. (F) The uptake of Alex647-WGA was quantified in cells expressing GFP or DN-dynaminK44A (K44A) and normalized to uptake in adjacent cells that did not express exogenous protein. Data are mean±s.e.m. (n⩾70).
Article Snippet: Other antibodies used in these studies include mouse monoclonal antibodies specific for caveolin-1 and -2,
Techniques: Quantitation Assay, In Situ, Expressing, Immunofluorescence